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The spICP-MS approach has several analytical constraints that must be considered in research and practical appliance. First of all, NPs are presumed as spheres in mathematical calculations, resulting in the biased perception of instances when non-spherical NPs are measured. Then, sample suspension attenuation requires estimations for establishing the most appropriate concentration for introducing a particle per a specific period in the plasma. Additionally, indication for the optimum concentration to provide the plasma with one particle at a time cannot be performed without multiple dilutions, a process that needs to be long and conscientious. Lastly, multi-element identification is impractical, yet the contents of each particle should be determined before the spICP-MS analysis. The spICP-MS is a highly-potent methodology for metallic NP characterization regardless of the constraints and aspects for improving the process includes.
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