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A light microscope is classic instrumental analysis equipment in biology that uses the properties of light to examine microscopic structures. In the optical microscope device, a beam of light hits a specimen located on a slide and then travels through a system of mirrors into the objective. The use of optical circuits inside the eyepiece allows a significant increase in the size of the observed image. It is noteworthy that the observed object reflects the light falling on it, so a light microscope can only observe objects that are large enough to reflect waves from the visible electromagnetic spectrum; it is impossible to use such a microscope to observe molecules or even more so atoms.
In addition, as part of the study, I understood exactly how to use the focusing image and what physical principle lies in it. More specifically, the focus screws allow the eyepiece device to be raised and lowered so as to achieve the most precise image possible. Focusing increases or decreases the distance between the objective lens and the slide glass on which the specimen being observed is placed. With this shift, the distance of the lens, which is responsible for focusing the reflected rays, changes, allowing the object to be observed either as straightforward or as too blurry.
On light microscopes, there are several lenses at once, which, through built-in lenses, allow differential magnification of the image. One of the main lessons I learned while studying this topic is to strictly warn against using a coarse focus when using the most powerful eyepiece. As the magnification turns out to be maximum, the eyepiece requires the maximum amount of incoming light, so the object itself is not only the largest but also the closest to the objective glass. If you use a coarse — vertical — focus on such a lens carelessly, it is not unlikely that the lens will damage the objective glass or vice versa. Thus, the main lesson in this topic concerns the limiting rules when using a light microscope and learning how it works. That said, the most exciting part of the study was the analysis of the physical concepts that underlie the use of such a microscope.
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