Do you need this or any other assignment done for you from scratch?
We have qualified writers to help you.
We assure you a quality paper that is 100% free from plagiarism and AI.
You can choose either format of your choice ( Apa, Mla, Havard, Chicago, or any other)
NB: We do not resell your papers. Upon ordering, we do an original paper exclusively for you.
NB: All your data is kept safe from the public.
E.coli has been found as a modal vector in which genes of different sources can be expressed. There have been many developments in the systems through which protein expression and purification can be achieved using E. coli as the cloning agent. Clontech HAT (Histidine Affinity Tag) is a protein expression and purification system whereby the vectors encode a novel polyhistidine epitope. This epitope allows the proteins that are expressed in bacteria to be purified at physiological pH at denaturing circumstances or conditions. This system presents two major advantages in that the proteins involved are soluble and hence they are no apparent aggregates within the inclusion bodies. The fact that the proteins can be washed out under neutral pH is also advantageous. (Scientifix, 2011)
Several purification strategies have also been devised. The clontech xTractor Buffer generally disrupts the bacterial cells to enhance protein purification. This system of protein purification has been optimized to enhance the extraction of poly-histidine tagged proteins. The extraction of protein using this method is simple. The cells are re-suspended in the buffer and mixed gently for about 10 minutes. The mixture of salts that results produces a lysate that has no visible precipitates. Proteins obtained through xTractor Buffer have a higher biological activity than cells that would be obtained through sonication. (Scientifix, 2011)
Other methods relate to protein expression and purification in E.coli and it is all a matter of choice depending on the characteristic of cell that one would want to achieve at the end of it. The choices are also dependent on the available time limit as well as financial resources at ones disposal. However, the methods are eligible and result-oriented.
References
Scientifix, 2011, Protein Expression and Purification in E.coli, Web.
Do you need this or any other assignment done for you from scratch?
We have qualified writers to help you.
We assure you a quality paper that is 100% free from plagiarism and AI.
You can choose either format of your choice ( Apa, Mla, Havard, Chicago, or any other)
NB: We do not resell your papers. Upon ordering, we do an original paper exclusively for you.
NB: All your data is kept safe from the public.